Infinite Enzymes’ MnP is a Manganese Peroxidase Enzyme (E.C. 18.104.22.168) from Phanerochaete chrysosporium, a white rot fungus. It is produced in a recombinant corn seed production system (Clough et al., 2006). It is provided as a lyophilized powder. Activity is determined using 2,6-dimethoxyphenol. Manganese peroxidase is an enzyme present in many fungi, but is particularly prevalent in wood rot fungi. It is a monomer of 53 kDa. Phanaerochaete chrysosporium is a fungus whose role is to degrade lignin in rotting trees (Jha and Patil, 2011).
MnP contains one heme prosthetic group per protein molecule (i.e., contains iron) and has the same catalytic cycle as other peroxidases. It involves the transfer of 2 electrons of the heme to H2O2 and subsequent reductions of the iron in the heme group in two 1-electron steps. The best reducing substrate for the enzyme’s heme group is Mn II. The Mn III formed by the cycle oxidizes other substrates like phenols and aromatic amines and is converted back to Mn II. This latter activity is the key to its utility in industrial applications—Mn III oxidation of phenolic compounds.
The enzyme should be stored as a dry powder at 4° C. After reconstitution, store at 4oC or -20oC for long periods.